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Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
Precision Plus Kaleidoscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad precision plus protein standards kaleidoscopetm marker
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
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Bio-Rad nitrocellulose membranes
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
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Bio-Rad sds page protein standards
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
Sds Page Protein Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad precision plus protein kaleidoscope ladder
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
Precision Plus Protein Kaleidoscope Ladder, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad kaleidoscope markers
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
Kaleidoscope Markers, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad kaleidoscope precision plus protein standards
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
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Thermo Fisher precision plus protein™ kaleidoscope standards
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
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Bio-Rad molecular marker precision plus protein standard kaleidoscope
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
Molecular Marker Precision Plus Protein Standard Kaleidoscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad kaleidoscope marker
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
Kaleidoscope Marker, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad precision plus protein kaleidoscope standard
Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored <t>Kaleidoscope</t> markers transferred to the blot.)
Precision Plus Protein Kaleidoscope Standard, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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Image Search Results


Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored Kaleidoscope markers transferred to the blot.)

Journal: Analytical Chemistry

Article Title: Thermostable Llama Single Domain Antibodies for Detection of Botulinum A Neurotoxin Complex

doi: 10.1021/ac8014774

Figure Lengend Snippet: Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored Kaleidoscope markers transferred to the blot.)

Article Snippet: Molecular weight standards were Precision Plus Unstained for the silver stain and Precision Plus Kaleidoscope for the Western blot (BioRad, Hercules, CA).

Techniques: Clone Assay, Staining, Molecular Weight, Western Blot