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Image Search Results
Journal: Analytical Chemistry
Article Title: Thermostable Llama Single Domain Antibodies for Detection of Botulinum A Neurotoxin Complex
doi: 10.1021/ac8014774
Figure Lengend Snippet: Figure 7. Epitope mapping the sdAb clones. Panel A shows silver- stained proteins of molecular weight markers (M), serotype A toxin (T), and toxin complex (TC) to confirm that all known components of the complex are resolved. Molecular weights in kDa are indicated on the right. Panel B shows toxin complex Western-blotted and probed with each of the sdAb clones or no sdAb (-ve) as fusions to hyperactive alkaline phosphatase, all appearing to bind a 33 kDa species. (Molecular weights in kDa shown on the right, were estimated from the colored Kaleidoscope markers transferred to the blot.)
Article Snippet: Molecular weight standards were Precision Plus Unstained for the silver stain and
Techniques: Clone Assay, Staining, Molecular Weight, Western Blot